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From the lab: Rabbit IgG competitive ELISAs with goat-bound to HRP as detect ready to train biochemists. Break out the multi-channel pipettes!
Before blotting, this is a measurement of purity by weight benchmarked by an antibody from ABcam.
Contained in the respective lanes of this gel are:
Lane 01: Molecular Weight Marker
Lane 02: Commercial Rabbit IgG ABcam
Lane 03: IGY CxHu 1/32 Dilution
Lane 04: IGY CxHu 1/16 Dilution
Lane 05: IGY CxHu 1/8 Dilution
Lane 06: IGY CxHu 1/4 Dilution
Lane 07: IGY CxHu 1/2 Dilution
Lane 08: IGY CxHu Undiluted
Lane 09: Molecular Weight Marker
- Since many of the standard adjuvants can cause biotoxic shock in even the hardiest birds, we are using a very simple method developed in Holland. It involves Specol and precursors to Freunds ajuvant.